secondary VHH

Just as traditional secondary antibodies amplify signals and expand the versatility of primary antibody detection, secondary nanobodies (VHHs) are revolutionizing protein analysis with enhanced performance and unique capabilities.

Secondary nanobodies, or Variable Heavy-chain Domain (VHH), offer a next-generation approach to this critical function. These single-domain antibodies, derived from camelids, target the host species or isotype of primary antibodies, or even specific tags on primary antibodies, with significant advantages, especially for high resolution imaging.

• Smaller Size & Improved Penetration: Their compact size (15 kDa) allows for superior penetration into tissues and cells, crucial for applications like super-resolution microscopy and high-resolution Immunohistochemistry and Immunofluorescence where precise localization is paramount. This also reduces steric hindrance, enabling better access to epitopes.
• High Affinity & Specificity: As exemplified by alpaca anti-mouse IgG1 Fc-specific recombinant VHHs, these nanobodies exhibit high affinity and specificity, leading to robust signal amplification, improving detection sensitivity, and reducing background. This allows for clear and precise signals.
• Enhanced Versatility & Labeling: Secondary nanobodies can be engineered with various labels (e.g., fluorescent dyes like Alexa Fluor 488, enzymes) or conjugated to other molecules, expanding their application range. They can be generated against a wide variety of primary antibody host species and isotypes.
• Improved Stability: Nanobodies are inherently stable, ensuring consistent performance across diverse experimental conditions.
• Reduced Background: Their high specificity minimizes non-specific binding, resulting in cleaner signals and improved data quality.

At Echo Bio, We provide high-performance secondary VHH targeting primary antibodies raised from diverse hosts (human, mouse, rat, rabbit, chicken, goat, sheep), across various serotypes. By leveraging our secondary nanobodies, you can achieve enhanced sensitivity, precision, and versatility in their protein detection and analysis workflows, particularly in high-resolution imaging applications, pushing the boundaries of scientific discovery.