Specification
| Modification | Cap1, m1Ψ |
| Length | 6652nt |
| Application | 1. Analytical or process development 2. In vitro or in vivo expression and distribution 3. Experimental control reference |
| Concentration | 1 mg/ml |
| Storage Buffer | 1mM Sodium citrate, pH6.5 |
| Storage Condition | -80℃ to - 65℃ |
| Shipping Condition | Blue Ice or Dry Ice |
Background
| Backround | Prime editing (PE) systems minimally consist of two components: a programmable DNA nickase fused to an engineered reverse transcriptase and a pegRNA. The PE2/PE3 mRNA sequence was from publication: Nelson, et al. Nat Biotechnology, 2022; 40: 402-410. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudo Uridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA. |
QC Data
| Note | Please contact us for QC Data |
| Product Image (Reference Only) |  |