Specification
| Source | Alpaca |
| Volume | 500uL |
| Ligand | Anti-GFP Nanobody |
| Binding Capacity | 10ul Anti-GFP beads suspension binds to 3 - 4ug recombinant GFP proteins |
| Bead Property | Agarose beads covalently coupled with VHH antibodies/ nanobodie |
| Bead Size | ~45um (cross-linked to 7.5% agarose beads) |
| Storage Buffer | 20% ethanol, 0.03% sodium azide |
| Storage Condition | 4℃ for 6 months (Do Not Freeze). Long term storage: -20℃ for 12 months |
| Application | IP/Co-IP/ChIP/RIP, affinity purificatio |
Background
| Background | VHH or Nanobody is the next generation monoclonal antibody, whichn is a Single domain antibody (sdAb) reserving only one single antigen recognizing domain from camelid heavy chain antibody. With size of only ~ 15 kDa, Nanobody dispay numberous advantages over conventional antibody and it's derivatives, such as higher tissue penetration capability, more accessble to hidden epitopes, higher stability(thermal and pH resistant), solubility (aggregation avoidance) and binding affinity. Fluorescent protein GFP frequently used as reporter and fusion tag. Anti-GFP Nanobody Beads are agarose beads covalently coupled with VHH antibodies/ nanobodies acquiring high specificity and affinity for GFP. These nanobeads can efficiently capture and separate GFP and GFP tagged proteins from cell crudes or other samples. |
QC Data
| Please contact us for specific QC data. |